Composite

Part:BBa_K1795020

Designed by: Andrew D Halleran, Michael C LeFew, Joseph L Maniaci, John P Marken, Caroline A Golino   Group: iGEM15_William_and_Mary   (2015-09-17)

CFP-LVA under promoter R0051

Cyan fluorescent protein with LVA fast degradation tag under the control of strong lambda cl regulated promoter R0051. This part has been visualized both via plasmid-based expression and genome integration expression. Confocal microscopy (60x oil immersion objective on a Nikon A1R confocal) shows strong fluorescent signal even for single copy number expression. See merged CFP/YFP image: [1]


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None